In many countries, contamination of surface and subsurface water resources threatens human and other organisms' life. Improper application of animal manures and sewage sludge is considered an important cause for the soil and water pollutions. Pathogenic bacteria, viruses, and protozoa are the significant case of animal and human death in many parts of the world. Animal manures can contain several human pathogens that can potentially contribute to surface and groundwater contamination when applied to agricultural soils. Transport of pathogenic bacteria has been recently considered as a major source of surface and subsurface water contaminations. Bacterial transport through porous media and the affecting factors are the subjects of many recent researches. Many factors affect the bacterial transport through the soil profile. Soil physical, chemical and biological properties such as soil water content, porosity, flow velocity, pore attributes, pH, organic matter and temperature, climate factors and the bacteria properties such as size, type and mobility, could influence the bacterial transport. Soil might act as a filter for bacteria. The high soil water content could facilitate the bacterial transport. Water flow velocity is also an important factor affecting the bacterial transport. Specific surface of soil particles also affects the bacterial retention in the soil. Net electrical charges of most bacteria are negative so that adsorption is greater at low pH values due to positive charges of soil surfaces in acidic conditions.CALCIUM carbonate (CaCO3) and CALCIUM SULFATE (CaSO4) are the two important soil minerals in arid and semi-arid regions of Iran and many countries. There was no information about specific influences of these minerals on the bacterial transport. This study was conducted to explore the impacts of these two minerals on Pseudomonas fluorescents transport through repacked mineral-sand mixtures under unsaturated water flow condition. The sand was collected from the North beach and was carefully washed with distilled water. The sand had no organic matter but had 7.5% CaCO3, 2.1 and 8.3 g kg-1 amorphous and crystalline Fe, and 0.32 and 1.1 g kg-1 amorphous and crystalline AI, respectively. The treatments consisted of CaCO3 (0, 5, 10 and 20 %w/w) and CaSO4 (0, 5 and 10 %w/w) which were mixed with the sand. The experiment was in factorial arrangement adapted to a completely randomized design with three replicates.